Rhodopsin and Halobacterium, Complete Papers 2016-02-07

[Mustafa Umut Sarac]

Mustafa Umut Sarac submitted a new resource:

(there was a url link here which no longer exists) - Rhodopsin and Halobacterium, Complete Papers

Rhodopsin and Halobacteria, Complete Papers

STEP 1

Rhodopsin is a protein which is erasable in PVA 1 million times and it can take pictures with up to 5000 lp/mm.



You can buy it ready or you can grow it. If you want to invest only 10 dollars , halobacterium salinarum is sold live in tubes at high school biology supplier.



Lets start from start of the whole process to make your film.



You bought halobacteria - or halobacterium salinarum - and you want...

(there was a url link here which no longer exists)

 

[Mustafa Umut Sarac]

Biosynthesis of Rhodopsin from Halobacteria

STEP 2

First post was about halobacteria growing.


This paper relates to biosynthesis of rhodopsin from halobacteria.

Biosynthesis of the Purple Membrane of Halobacteria




By Manfred Sumper, Heribert Reitmeier, and Dieter Oesterhelt[*j
Dedicated to Professor Feodor Ljinen on the occasion of his 65th birthday
Halobacteria are extremely specialized organisms. They live exclusively in saturated solutions
of common salt. The cell membrane of these bacteria exhibit insular regions which can be
isolated by membrane fractionation. These regions consist of a lipid matrix containing bateriorhodopsin
molecules in a hexagonal crystalline arrangement. Bacteriorhodopsin is a deep purple
retinal-protein complex (“purple membrane”). The purple membrane functions as a light energy
converter. --How can such a differentiated membrane region arise? In uiuo studies on the
biosynthesis of the purple membrane showed another cell membrane fraction, the so-called
brown membrane, to be a biosynthetic precursor. Bacterioopsin (the retinal-free protein) is
initially incorporated into the brown membrane and can only form the purple membrane
by crystallization in an energy-dependent reaction after prior reaction with retinal. This reaction
is reversible. Removal of the retinal by formation of retinal oxime causes the purple membrane
regions to disappear. Reconstitution of the bacteriorhodopsin by addition of retinal regenerates
the purple membrane.​

 

[Mustafa Umut Sarac]

Making film out of rhodopsin

STEP 3

You bought bacteria,grew it, synthesized it , now its time to make a film with your rhodopsin.

Bacteriorhodopsin oriented in polyvinyl alcohol films as an erasable optical storage medium​

Zhongping Chen, Aaron Lewis, Hiroyuki Takei, and Isaiah Nebenzahl
Films of oriented bacteriorhodopsin have been formed in polyvinyl alcohol with excellent optical quality.
Images with high contrast have been impressed and erased on these films. Second-harmonic microscopy has
been used to read the image on a bacteriorhodopsin-polyvinyl alcohol film without erasure. The potential of
these films for molecular information storage and computation is discussed.
Keywords: Erasable optical storage, bacteriorhodopsin, biomolecular electronic devices​

 

[Mustafa Umut Sarac]

Making 3D Optical Storage and knowing rewrite characteristics of RD

STEP 4

You completed the film and want to learn all about writing, erasing, rewriting characteristics of rhodopsin. This paper is a must and in 4 parts.

PART 1

AFRL-IF-RS-TR-2002-319
Final Technical Report
January 2003
PROTOTYPE PROTEIN-BASED THREEDIMENSIONAL MEMORY
Syracuse University
​

 

[Mustafa Umut Sarac]

PART 2

PART 2

AFRL-IF-RS-TR-2002-319
Final Technical Report
January 2003
PROTOTYPE PROTEIN-BASED THREE DIMENSIONAL MEMORY
Syracuse University

 

[Mustafa Umut Sarac]

PART 3

PART 3

 

[Mustafa Umut Sarac]

PART 4

PART 4

 

[AgX]

I regard bio-sensors as analogue.

 

[BobCrowley]

I think this is genius. The eye uses rhodopsin (which means purple) and a chain of chemical events excite the neurons. Here it can be made into a biofilm and potentially be used as a photographic recording system of incredible sensitivity and resolution! It most certainly falls into the so-called analogue category to me.

 

[Rick A]

As this is not analog, but rather biological, it should be posted on APUG's sister site, BPUG.

+1

 

[stormpetrel]

Very interesting indeed! I will read all the papers carefully when I will have a moment.

 

[Truzi]

As this is not analog, but rather biological, it should be posted on APUG's sister site, BPUG.

I get your joke, but... gelatin is/was biological, so would we need finer granularity than merely bio-PUG.