The diameter of the aperture and the FL of the optic does not usually change (for discussion purposes, ignoring AF macro lenses whose FL *does* change for macro distances (like the Canon 50mm EF macro) so the f/stop is NOT altered. The reason for apparent loss of light at macro distance is simply that the photon falling within the image circle are SPREAD OUT over a LARGER AREA when the image is focused for macro distances, thus the image at the film plane is dimmed in intensity by that greater area, rendering the f/stop to be APPARENTLY smaller than the set number.If you go as far as 1:1, the f/number at each side is now twice what it says on the lens scale.
Hence if you shoot at 1:1 at a marked f/number of f/16, it is like shooting at f/32 in terms of resolution and detail contrast ( MTF ) .
The diameter of the aperture and the FL of the optic does not usually change
The lens focal length is still 'f' but 1/f = 1/u + 1/v
Yes, I agree with you on all that.
My point was that once you operate at finite distance, you now have two focal distances, 'u' and 'v' , each of which has its own f/number.
In the simple example of 1:1 , both 'u' and 'v' are now 100mm.
If your lens is still set at f/2, then the aperture is 25mm, and the f/number at each side if f/4.
This goes with the reduction in brightness, as you point out.
The lens conjugate equation ( above ) can help give you the f/number at each side, at any other magnification.
If you know the f/number then you can also predict the diffraction cut-off frequency, if you want.
Otherwise just refer to what you know about what minimum f/number is acceptable on your format.
Mark,
... so if you can point to any academic evidence as proof of that concept, I would be happy to reconsider.
Some of this might also depend upon the lens you are using. The SK literature for their makro symmar HM lenses recommends smaller apertures for greater magnification which seems somewhat counterintuitive.
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