Aerobiologia 16: 441448, 2000.
© 2000 Kluwer Academic Publishers. Printed in the Netherlands.
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Aerobiological monitoring of the Sistine Chapel: airborne bacteria and
microfungi trends
Riccardo Montacutelli1, Oriana Maggi2, Gianfranco Tarsitani1 & Nazzareno Gabrielli3
1Istituto di Igiene, Universit`a La Sapienza, P.le Aldo Moro 5, I-00185 Rome, Italy; 2Dipartimento di Biologia
Vegetale, Universit`a La Sapienza, Rome, Italy; 3Gabinetto di Ricerche Scientifiche dei Musei Vaticani Stato
Citt`a del Vaticano, Italy
(Author for correspondence)
Received 2 March 1999; accepted in final form 16 may 2000
Key words: aerobiological monitoring, bacteria, microfungi, Sistin Chapel
Abstract
The present paper reports the results of a bacteriological and mycological monitoring carried out on the airborne microflora of the Sistine Chapel. The general aim of the study was to evaluate the impact of the flow of visitors, as well seasonal effects, on the qualitative and quantitative variations of microorganisms. Two sampling campaigns were carried out in May and November 1997. A Surface Air System (SAS) sampler (active system) and a sedimentation based sampler (passive system), supported by an original plinth, were used. Temperature, humidity and carbon dioxide were detected. VITEK SYSTEMS jr. for Staphylococcus spp. and microscopic observation for microfungi were the identification methods. In spite of the conditioning and filtration air system, initial results with both samplers, show a positive correlation between the airborne microorganisms and presence and number of visitors. The SAS samples showed higher microbial load, for both bacteria and fungi, than the passive ones, but the epidemiological meaning of the differently collected data varies. The increase during visiting hours of human Staphylococcus spp. is stronger than the airborne bacterial load increase. The microfungi most frequently isolated were Cladosporium spp. and Penicillium spp. These preliminary data underline the significance of the survey for the protection of such a precious environment, and encourage the Authors to continue the ongoing monitoring.