One consideration that is usually neglected in most DOF discussions is that the CofC used in the calculation is typically something appropropriate for somewhat less than perfect vision...someone with 20/20 vision (as the optometrist in the US will correct your vision), can see CofC of that size on a 8x10" print viewed from 12" distance as blur circles and NOT perceive them as in focus 'points'.
The CofC size for 20/20 vision has to be SMALLER than typically assumed in most DOF calculators! The DOF zone depth is shallower when assuming 20/20 vision of the viewer, compared to the looser 'manufacturer standard' CofC.