For films you need just enough to break the surface tension of the water and prevent it from beading. Try 1 drop for 250 ml of water to start.
Kodak Photo-Flo uses a different chemical called Triton X-100. I mention it because it is not readily mscible with water and must be diluted with propylene glycol. Don't know about Tween 20 and it's miscibility. In the past X-100 was also sold as a solution in isopropyl alcohol for photo purposes.
I have used a drop of it (dip tip of eye dropper into tween bottle; don't squeeze bulb or you will spend forever trying the rinse it out of the insides of the dropper) ) into about 20mL of cyanotype solution. I mixed it in just prior to coating, when I am coating with a foam brush. I find it results in fewer bubbles.