I actually experimented with this quite a bit.
I used MagnaSight and MicroSight for this test. MagnaSight is an image focuser with X8 magnification and MicroSight is an grain focuser with X25 magnification. I was using f/5.6 which is wider than my usual f/8 or f/11. I can bring the image to sharp focus, then lower or raise the focuser about 1/4" without seeing any difference. This test was done using both 35mm and 645 negatives projected to 8x10 equivalent size.
Go ahead and try it.... use a notebook or something and white sheet. Focus, then remove the notebook. Focus then add another notebook. See if there are any difference. I couldn't see any.