Not with NikonScan. To get the most out of your negs and slides you'll need VueScan. VueScan pulls a lot more shadow and highlight details in single pass mode than NikonScan.
I used to scan slides (Fuji Provia 100F) I've shot with super sharp Zeiss lenses with NikonScan and had to use 4x multisampling to get the most out of them. Then I used VueScan and - bingo - got a really sharp scan with single pass and a much better dynamic range.
Just make sure to calibrate the scanner every 30 minutes.
NikonScan produces pretty sharp edges in color blends and banding when scanning shadow areas, even @ 16 bit color depth saved as *.tif.
Both Nikon scanners are fantastic - they just need a good software. For me scanning with VueScan put my Nikons on steroids, really. A lot faster, sharper and with a much better color rendition.
Just download both - Silverfast and VueScan - and run some test. Don't forget to enable the advanced menus in VueScan. VueScan costs a fraction of Silverfast and with the Pro version you'll get life long free updates compared to Silverfast, where you have to churn out some funds every now and then.
The big advantage of VueScan is that you can use it with **any** scanner on their list. And with **any** widespread OS currently on the market: Linux, Windoze, Mac.
just my 2 cents...