Try printing it again first. If it shows enough to ruin the print, then I would try soaking the negative in some alkaline solution... washing soda (sodium carbonate), borax, Dektol, etc., followed by another wash in water. Cyanotypes bleach in alkaline solutions so it might remove the stain. If the Prussian Blue has somehow reacted with the silver of the negative, it might remove part of the image too, but I've never heard of that actually happening. I suppose it would be possible since potassium ferricyanide, a silver bleach, is on of the components of the original cyanotype solution.