Yes each lens, each set up, each lighting change, etc. It's kind of a moving target with camera capture because the conditions can change so much from shot to shot. You may be able to get close if you do one for each lens and each type of lighting, but to really get the types of results that some people are looking for you need to almost have the chart in each shot. The bigger the chart (more patches) the more relevent this becomes. The 24 patch is a pretty wide correction and will probably give you good results over a wider range of conditions.